Once the eggs are retrieved they are identified under the microscope. Each egg is typically surrounded by cells. The embryologist will “strip” the cells away from the egg. Sperm is also prepared for fertilization by “washing” the sperm. This process removes non-motile sperm and seminal fluid. Once the preparation process is complete the sperm and egg are brought together. There are two methods in which this can be performed. The first method is an incubation method; where approximately 75,000 sperm are incubated with each egg in culture media. 18 hours later the embryologist evaluates the eggs to visualize if fertilization occurred. The second method utilized is ICSI (intracytoplasmic sperm injection), Where a single sperm is selected and injected directly into the egg.
Egg after stripping ICSI Fertilization occured IVF Microscope